Read mRNA Processing and Metabolism Methods and Protocols (Methods in Molecular Biology)
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Published on: 2004-02-05
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Original language: English
Cells possess a wealth of posttranscriptional control mechanisms that impact on every conceivable aspect of the life of an mRNA. These processes are intimately intertwined in an almost baroque manner, where promoter context influences the recruitment of splicing factors, where the majority of pre-mRNAs undergo alternative splicing, and where proteins deposited during nuclear processing impact distal cytoplasmic processing, translation, and decay. If there is a unifying theme to mRNA Processing and Metabolism: Methods and Protocols, it is that mRNA processing and metabolism are integrated processes. Many of the techniques used to study mRNA have been described in a previous volume of this series (RNA–Protein Interaction Protocols, Susan Haynes, ed.) and specialized methods journals. In selecting topics for mRNA Processing and Metabolism: Methods and Protocols, I sought input on new and novel techniques and approaches that build on this foundation using technological advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference. I have tried not to bias this book toward any single model organism, and approaches described in the various chapters use yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells. Current Protocols in Molecular Biology - Wiley Online Library Archive Current Protocols in Molecular Biology Supplements. 2017: Supplement 117 2016: Supplement 116 Supplement 115 Supplement 114 Supplement 113 Electroporation Publications / Protocols - Sonidel Limited Electroporation Publications. CUY21 Electroporator Range [2007] Hepatocyte Growth Factor Promotes Colonic Epithelial Regeneration via Akt Signaling Transcriptomics Journals High Impact Articles list NLM ID: 101620306. Transcriptomics is a total set of transcripts in a given organism and referred to as an expression profiling that examines the expression level of ... Methods in Enzymology - ScienceDirect.com Methods in Enzymology Volume 584 Pages 2-474 (2017) Enzymology at the Membrane Interface: Intramembrane Proteases RNA Interference: Biology Mechanism and Applications INTRODUCTION. RNA silencing is a novel gene regulatory mechanism that limits the transcript level by either suppressing transcription (transcriptional gene silencing ... Program Schedule International Society Hide All; Union Biometrica; STEMCELL Technologies; Thermo Fisher Scientific; Automation for Analysis and Handling of Cells and Cell Clusters in Stem Cell Research. Metabolomics - Wikipedia Metabolomics is the scientific study of chemical processes involving metabolites. Specifically metabolomics is the "systematic study of the unique chemical ... DNA sequencing - Wikipedia DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine ... Molecular Biosciences Ph.D. Middle Tennessee State ... The Molecular Biosciences Ph.D. interdisciplinary program focuses on the study of biological problems at the molecular level using chemical ... Biology - Open Access Biological Sciences Journal Biology an international peer-reviewed Open Access journal. ... In dividing vertebrate cells multiple microtubules must connect to mitotic kinetochores in a highly ...
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